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K. Kosuge N. Watanabe T. Kuboyama V. M. Melnik V. I. Yanchenko M. A. Rosova N. P. Goncharov 《Euphytica》2008,159(3):289-296
Two mutants for sphaerococcoid seed (MA 16219) and compact spike (MA 17648) were isolated from M3 progeny of durum wheat cultivar, Altaiskaya Niva, mutagenized with chemical mutagens. The chromosomal locations of the genes
involved were determined by the use of a complete set of D-genome disomic substitutions in durum cultivar, Langdon. The gene
for sphaerococcoid grain, s
16219
, was allelic to S2, located in the centromeric region of chromosome 3B in hexaploid wheat. The gene for compact spike, C
17648
, was located on chromosome 5AL distal to the Q locus. Using microsatellite markers, C
17648
and awn inhibitor B1 were located in the F2 of LD222 × MA17648. The gene order was Xbarc319—C
17648
—Xgwm179—Xgwm126—Xgwm291—B1. 相似文献
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Isolation of Encephalitozoon cuniculi using primary tissue culture techniques from a rabbit in a colony showing encephalitozoonosis 总被引:1,自引:0,他引:1
Furuya K Fukui D Yamaguchi M Nakaoka Y Bando G Kosuge M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(2):203-206
Encephalitozoon spores were isolated in a primary tissue culture of the kidneys from an encephalitozoonosis-suspected rabbit in a municipal zoo in Hokkaido. The isolated spores were morphologically characteristic of microsporidial ones in chromotrope stain, and proven to be E. cuniculi by a polymerase chain reaction (PCR) with a species-specific primer set and by direct DNA sequencing of the PCR products. 相似文献
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